Details
Product Name | F(ab')₂ Fragment Goat Anti-rabbit IgG H&L / Biotin |
Chinese Name | 生物素标记的F(ab)2片段羊抗兔IgG H&L抗体 |
Alias | F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (Biotin); F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/Biotin; F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (Biotin); Biotin AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L); |
Immunogen Species | Goat |
Clonality | Polyclonal |
React Species | Rabbit, |
Applications | IHC-P=1:400-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
Form | Liquid |
immunogen | Native Rabbit IgG |
Lsotype | IgG |
Purification | affinity purified by Protein G, nonspecific adsorbed |
Buffer Solution | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
Attention | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
Product Detail |
Immunoglobulin G (IgG) is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
Product Picture |
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACE2) Polyclonal Antibody, Unconjugated (SL46001R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining.
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Partial purchase records (15)
Username | Quantity | bought time |
Le*** | 3 | 2024-03-28 |
Ad*** | 1 | 2024-02-15 |
Ka*** | 3 | 2023-12-27 |
Sa*** | 2 | 2023-12-02 |
Di*** | 3 | 2023-09-23 |
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