Drug Names

Generic Name：Fish Brain derived neurotrophic facor(BDNF) ELISA Kit

Purpose

Our Fish Brain derived neurotrophic facor(BDNF) ELISA kit is to assay BDNF levels in Fish serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to BDNF. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for BDNF is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain BDNF and HRP conjugated BDNF antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BDNF. You can calculate the concentration of BDNF in the samples by comparing the OD of the samples to the standard curve.