Drug Names

Generic Name：Canine Tumor necrosis factor γ(TNF-γ) ELISA Kit

Purpose

Our Canine Tumor necrosis factor γ (TNF-γ) ELISA kit is to assay TNF-γ levels in Canine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TNF-γ. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TNF-γ is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNF-γ and HRP conjugated TNF-γ antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-γ. You can calculate the concentration of TNF-γ in the samples by comparing the OD of the samples to the standard curve.