Drug Names

Generic Name: Bovine Glutamate Dehydrogenase (GDH/GLDH)ELISA Kit

Purpose

Our Bovine Glutamate Dehydrogenase (GDH/GLDH)ELISA kit is to assay GDH/GLDH levels in Bovine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GDH/GLDH. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GDH/GLDH is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GDH/GLDH and HRP conjugated GDH/GLDH antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GDH/GLDH. You can calculate the concentration of GDH/GLDH in the samples by comparing the OD of the samples to the standard curve.