Drug Names

Generic Name: Rat Cross Linked C-Telopeptide Of Type I Collagen (CTX-I)ELISA Kit

Purpose

Our Rat Cross Linked C-Telopeptide Of Type I Collagen (CTX-I)ELISA kit is to assay CTX-I levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CTX-I, Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for CTX-I is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CTX-I and HRP conjugated CTX-I antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CTX-I. You can calculate the concentration of CTX-I in the samples by comparing the OD of the samples to the standard curve.