Drug Names

Generic Name: Mouse Nuclear Factor Kappa B (NF-kB)ELISA Kit

Purpose

Our Mouse Nuclear Factor Kappa B (NF-kB)ELISA kit is to assay NF-kB levels in Mouse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NF-kB. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for NF-kB is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NF-kB and HRP conjugated NF-kB antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NF-kB. You can calculate the concentration of NF-kB in the samples by comparing the OD of the samples to the standard curve.