Product Introduction:

Coomassie G-250 dye binds to proteins in acidic solution, increasing the maximum absorption peak (Imax) of the dye from 465nm to 595nm. Within a certain concentration range, the measured absorbance value A595 is proportional to the protein concentration. The Bradford method determines protein concentration unaffected by chemicals present in most samples. The concentration of mercaptoethanol in the sample can reach up to 1M, and dithithuritol up to 5mM. However, due to slightly higher concentrations of descalers, it is necessary to ensure that SDS concentration is below 0.1%, Triton X-100 below 0.1%, and Tween 20, 60, 80 below 0.06%. For samples containing descaling agents, it is recommended to use a BCA protein concentration determination kit produced by Sunlong Biotech.