Measurement

: The mitochondrial complex IV is also known as the cytochrome C oxidase, is also a common component of the main road and the branch of the mitochondrial breathing electron transfer chain, responsible for catalysisThe oxidation of the reduced cytochrome C and finally transmits electrons to oxygen and produces water.

Reductive cytochrome C at 550 nm with a characteristic light absorption, mitochondrial complex IV catalytic reduction type cytochrome C generates oxide cytochrome C, so 550 nm light absorption decline rateIt is possible to reflect the IV activity of mitochondrial complex.

Instruments, consumables: visible spectrophotometer / enzyme, desktop centrifuge, water bath, adjustable pipette, trace quantum-based cattle / 96-well plate,Drug, ice and distilled water.