Measurement Significance:

Glycogen is a polymer polysaccharide composed of glucose units, one of the main storage forms of sugar, mainly stored in the liver and muscles.As the spare energy, it is called hepatic candies and muscle glycogen, respectively.The hepatic sugar can regulate blood sugar concentration. When blood sugar is increased in the liver synthesis of glycogen, when blood sugar is lowered, the principle of hepatose is decomposed into glucose to supplement blood sugar.Therefore, the relative balance of hepatose is very important for maintaining blood sugar.The muscle glycogen is a storage form of sugar in muscles. When consuming a lot of blood sugar vigorously, the muscle glycogen cannot directly decompose into blood sugar, which must first decompose to produce lactic acid, recycled to the liver with blood, and transformed into a hepatic candy orglucose. Determination Principle:

Anthrynone method.The glycogen was extracted using a strong alkaline extract, and the glycolin content was determined using an anthrazone selection under strong acid conditions.

Required instruments, supplies:

visible spectrophotometer / enzyme, water bath, adjustable pipette, trace quantine biopsy / 96-well plate, concentrated sulfuric acid (noAllow express) and distilled water.