Determination principle:

PFP catalytic 6-phosphate fructose into 1,6-di phosphate jug, it is in aldolase and phosphosome isomerase The action was transformed into 3-phosphoric acid glyceryl, and then 3-phosphate dehydrogenase and NADH catalyzed 3-phosphouric acid, NAD and phosphoric acid, and the absorbance variation at 340 nm reflected the activity of PFP. Need instruments, consumables: balance, low temperature centrifuge, mortar, ultraviolet spectrometry / enzyme symbol, trace quantity-free cuvette / 96-well plate.

Determination significance:

Pyphosphate: fructose-6-phosphate-1-phosphate (PFP, EC2.7.1.90) is a cytoplase, widely found in plant tissue In the reversible conversion of catalytic fructose-6-phosphate and fructose-1,6-diphosphate, it plays an important role in photochariting carbon metabolism.

Scheme:

PFP catalytic 6-phosphate jug of 1,6-di phosphate jug, is converted to 3- The phosphorched aldehyde was then catalyzed from 3-phosphate dehydrogenase and NADH to form 3-phosphouric acid, NAD and phosphoric acid, and the absorbance variation at 340 nm reflected the activity of the PFP. Need instruments, consumables: balance, low temperature centrifuge, mortar, ultraviolet spectrometry / enzyme symbol, trace quantity-free cuvette / 96-well plate.

\u0026 nbsp;