3-Phosphoglycerate Kinase (PGK) Activity Assay Kit        Note: It is necessary to predict 2-3 large difference samples before the formal determination. Operation Equipment: Spectrophotometer/microplate reader

Cat No: AK0393

Size: 100T/96S

Product Description:

PGK is the key enzyme of glycolysis. It's also a key enzyme for organisms to survive. It widely exists in animals, plants and microorganisms. It has many biological functions, such as affecting DNA replication and repair, stimulating RNA synthesis of virus, and is widely used in drug target design.

PGK catalyzes the production of 1,3-diphosphoglyceride and ADP from 3-phosphoglycerate and ATP. 1,3- diphosphoglyceride produces 3-phosphoglyceraldehyde, NAD and phosphoric acid under the action of 3- phosphoglyceraldehyde dehydrogenase and NADH. The absorbance decreased at 340nm. It reflects the activity of 3-phosphoglycerate kinase.