Purpose

Our Human Lambda Light Chain,λ-IgLC ELISA Kit  is to assay soluble terminal complement complex λ-IgLC levels in Human  serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to soluble terminal complement complex λ-IgLC . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for soluble terminal complement complex λ-IgLC  is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain soluble terminal complement complex λ-IgLC  and HRP conjugated soluble terminal complement complex λ-IgLC  antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of soluble terminal complement complex λ-IgLC . You can calculate the concentration of soluble terminal complement complex λ-IgLC  in the samples by comparing the OD of the samples to the standard curve.