Kit Performance

Physical Performance: 

All liquid components should be clear and transparent, without precipitation or flocculent substances. The microplate aluminum foil bag should be vacuum-sealed without damage or leakage.

Standard Curve Linearity: 

The correlation coefficient (r value) of the calibrator dose-response curve should be ≥ 0.99.

Precision: 

Intra-assay coefficient of variation (CV%) is less than 10%;

Inter-assay coefficient of variation (CV%) is less than 15%.

Sensitivity: The minimum detectable dose is less than 46.875 pg/mL.

Recovery Rate: Recovery rate is between 85% and 115%.

Specificity: 

This kit recognizes both natural and recombinant Reverse Triiodothyronine (rT3). No cross-reactivity with structurally similar compounds.

Stability: Store at 2-8°C. The validity period is 6 months.

Detection Range: 375 pg/mL-6000 pg/mL.

Intended Use:

For the determination of Reverse Triiodothyronine (rT3) concentration in samples such as serum, plasma, and cell culture supernatant.

Assay Principle

This kit employs a competitive ELISA method. Samples/calibrators and Assay Diluent are added sequentially to the microplate wells pre-coated with Reverse Triiodothyronine (rT3) antibody. After incubation and washing, SA-HRP is added and incubated. Following another wash, the substrate TMB is added for color development. The reaction is stopped with stop solution after color development at room temperature, and the Optical Density (OD) is measured. The OD value is inversely proportional to the Reverse Triiodothyronine (rT3) concentration in the sample.

This kit features high sensitivity, strong specificity, good reproducibility, simple and quick operation, and provides reliable detection performance for decreases or increases of rT3 in serum.