Product Introduction:

The methods of DNA staining include Feulgen method, Methyl Green-Pyronin method, Acridine Orange Fluorescence method and so on. The most classical one is Feulgen method, which is a classical enzyme histochemistry method.
The principle of this kit is that after DNA is hydrolyzed by mild weak acid (such as hydrochloric acid), the glycosidic bond between purine base and deoxyribose is opened, and the phosphoester bond between deoxyribose and phosphoric acid is broken, forming free aldehyde group at one end of deoxyribose. The aldehyde group combines with Schiff Reagent in situ to form a purplish red compound, which makes the part containing DNA in the cell show purplish red. The reason of purplish red is that there is a quinone group in the molecule of the reaction product (the quinone group is a chromophore with color, so the part containing DNA is purplish red). This hydrolysis does not affect the ribose-purine binding bond, so RNA will decompose after being treated with this method, so this method is not applicable to prove RNA.

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Experiment picture

Paraffin section of mouse small intestine
Intracellular DNA stains purplish-red.