Product Introduction:

Calcium is abundant in the human body and forms the skeleton as a scaffold to support the human body. It also plays an important role in secretion, transport, muscle contraction, and nerve conduction. Calcium exists in two forms in the body. One is ionized calcium, which exists in the blood circulation, the so-called blood calcium; The other binds calcium, which binds to proteins, carbonic acid, or phosphoric acid and is deposited in tissues. "Calcium normally permeates all tissues and cells, except bones and teeth, and generally does not appear in the tissue in a solid state." However, in some cases, calcium precipitates into solid and precipitates in tissues, which is called pathological calcium salt deposition. The deposited calcium salts are mainly calcium phosphate, followed by calcium carbonate. Calcium salts are usually monorefringent, but calcium oxalate is birefringent.

Many dyes can form chelates with calcium, including alizarin red S, rhodopsin, nuclear fast red, etc. Alizarin red S, an anthraquinone derivative, is a sodium salt of alizarin sulfonate, which can chelate with calcium salts in calcium carbonate or calcium phosphate to form orangi-red complexes. In general, these dyes are more effective in identifying moderate to large amounts of calcium than slight staining of trace calcium deposits. However, alizarin red S often yields more reliable results for a small amount of sediment. The common methods for calcium salt staining include silver nitrate method and alizarin red S method.

Use the modified McGee-Russell method in this staining solution, which is mainly composed of alizarin red S and Mayer hematoxylin. The combination of calcium salt and alizarin red S formed orange red, and the nucleus stained with hematoxylin is blue. It is especially suitable for staining tissues with small amount of calcium salt.

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Experiment picture

Paraffin section of fetal rat
Calcium salts stain red; cell nuclei stain blue.