Product Introduction:

The Prussian Blue Reaction is a method of staining non heme iron (containing heme, iron transporters, iron deposition, etc.) in cells or tissues containing granular iron. It plays an important role in the validation of diseases such as ischemic anemia, hemolytic anemia, hemochromatosis, and abnormal hemoglobin metabolism.However, due to the principle of dyeing, for iron content of below 30 μM/g is difficult to form a visible positive mark with the naked eye, but can be used in conjunction with other enhancers for the detection of iron death models.
The Prussian blue reaction was first proposed by Perls and is therefore most commonly referred to as the Perl iron staining method. However, since its proposal in 1867, numerous chemists and pathologists have optimized this reaction, among which the Lillie method, which is more widely used and well optimized, can label divalent and trivalent iron separately. This kit is a ready to use Lillie method ferric iron staining kit that uses nuclear fast red staining solution for contrast staining can effectively distinguish between hemosiderin and other pigments, with stable reagents and good staining specificity.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Experiment picture

Paraffin section of mouse spleen
Hemosiderin stains blue; cell nuclei stain red; background stains light red or is unstained.