Product Introduction:

Monoamine oxidase (MAO), including MAO-A and MAO-B, is a flavoprotein that binds to the outer membrane of mitochondria and can catalyze the oxidative deamination of neurotransmitters and biogenic amines. Monoamine oxidase is related to the aging of the body and is considered as a sign of aging. It mainly exists in various organs of vertebrates, especially in the secretory glands, brain and liver. Significant changes can be detected in depression, redox stress myocardial injury and tissue fibrosis. At the same time, it also catalyzes the metabolism of monoamines in plants such as invertebrates and bean buds, with low content and important physiological functions.

Monoamine Oxidase (MAO) Stain Kit (NBT Method) is mainly composed of MAO Fixative and MAO Staining Solution. It uses tryptophan salt as the substrate, and nitrotetrazolium salt as the hydrogen receptor. During the process of oxidative deamination, the hydrogen receptor is reduced in situ at the enzyme active site to dimethyl carbamate pigment precipitation. This reagent is only used for scientific research, not for clinical diagnosis or other purposes.

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Experiment picture

Frozen section of mouse intestine With counterstaining, MAO (monoamine oxidase) active sites show blue-violet precipitate; cell nuclei counterstained with methyl green appear green.,

Experiment picture

Frozen section of mouse intestine Without counterstaining, MAO active sites show purple precipitate; background and cell nuclei are colorless.