Product Introduction:

Acid phosphatase (ACP) is widely distributed in various tissues, mainly in the lysosome, so it is often used as a lysosome marker enzyme. The acid phosphatase outside of lysozyme exists in endoplasmic reticulum and cytoplasm. Acidic phosphatase is different in all kinds of animals. The optimum pH for its activity is 4.5-5.5.
The reaction principle of Acid Phophatase Stain Kit (Kaplow's/Azo Coupling Method) is that phosphoric acid and naphthol are released by hydrolysis of acid phosphatase with AS-BI as substrate at acidic pH. Naphthol was coupled with diazo salts to form colored products, which were localized in the cytoplasm. It usually used for fresh blood smear, cell smear, frozen section and paraffin section.

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Experiment picture

Paraffin/frozen section of mouse lung
Positive sites stain purplish-red; left shows paraffin section staining result, right shows frozen section staining result; both counterstained with methyl green.