Product Introduction:

This kit is not used to display alkaline phosphatase activity by metal precipitation method, but by azo coupling method (also known as simultaneous coupling method). Its principle is that under the alkaline condition of pH 9.2-9.8, alkaline phosphatase in cells can hydrolyze AB-BI phosphate to release phosphoric acid and naphthol, the latter can generate colored products with coupling diazonium salt, which is located in cytoplasm.

The staining solution is specially used for alkaline phosphatase staining of neutrophils in blood or bone marrow cell smear, and the result is more reliable than that of metal salt precipitation method.

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