Product Introduction:

Plant tissues produce various reactive oxygen species (ROS) under stress environmental conditions, and ROS activity is very high and extremely unstable. Therefore, the detection of ROS is usually determined by its final product. Superoxide anions are one of the main components of plant reactive oxygen species, belonging to an oxygen-containing free radical that can reduce NBT (Nitrotetrazolium Blue) to insoluble blue metal compounds, thereby locating superoxide anions in tissues.

The Solarbio Plant Tissue ROS Detection Kit(NBT Method) is used for superoxide anion staining in plant living tissues. It is generally used for overall staining of tender root tips, leaves, etc. After staining, the areas where superoxide anions accumulate are blue to dark blue.

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Experiment picture

Hibiscus syriacus leaf
Reactive oxygen species (ROS) deposition sites show blue precipitate.