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Differentiation refers to the process of removing the excessively combined staining solution with some specific solution after tissue dyeing. This process is called differentiation, and the solution used is called differentiation solution. In HE staining and other staining,weak acid solution is often used as the differentiation solution, because acid can destroy the quinone structure of hematoxylin, make the tissue and pigment separate and fade, and then carry out eosin staining, so as to ensure the clear staining of nucleus and cytoplasm.
ematoxylin-Eosin (HE) Differentiation Solution is commonly used for differentiation after hematoxylin staining, and it is recommended to differentiate within 5-15 seconds.
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