Product Information

Intended Use

Enzyme immunoassay for the quantitative detection of Sheep CD4 antigen in serum, plasma, culture media, or any biological fluid.

Principle 

The quantitative detection of Sheep CD4 antigen is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Sheep CD4. The corresponding antigen in the sample or standards binds the immobilized antibody after incubation. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate specific to CD4 is added, which binds the captured antigen, forming antibody-antigen-enzyme-labeled antibody complex upon incubation. In a second wash step, the unbound conjugate is removed.  The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to the standard curve.