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Purification magnetic beads of Strep-tag II protein

Purification magnetic beads of Strep-tag II protein
Total

Product Summary

Alias:Strep-tag II蛋白纯化磁珠,Strep-Tactin

NO.:60818

concentration:10%(v/v)

Storage: 2-8℃

Mean particle size:30-150 μm

    Number:

Details

The Strep Tactin beads based on superparamagnetic beads are designed for rapid and efficient separation Strep(II)-tag protein. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). Generally, it does not interfere with folding or bioactivity. Thus, there is no need for removing the tag. Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. The proteins obtained are bioactive and display a very high purity (above 99%).

 

FEACTURES
1. High capability of antibody binding.
2. Time-saving, simple and mild.
3. Obtained high-purity protein by one-Step.
4. No need removing the tag.

SPECIFICATIONS

Designation Sunlong Biotech™  Magrose Strep-Tactin
Bead Diameter (µm) 30μm~150μm
Ligand density ~6mg Strep-Tactin /mL Gel
Protein Binding Capacity ~7mg Strep-tag II protein/mL Gel
Slurry Concentration 10%(v/v)  Suspension
Storage solution 1×PBS(0.1%Tween-20+0.05%NaN3)
Storage temperature 2 ~ 8 °C for long; NO FREEZE
Binding/Washing Buffer 10mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH8.0
Elution Buffer 2.5mM desthiobiotin in Binding Buffer
Regeneration Buffer 0.5M NaOH or 1mM HABA

 

Application examples


SDS-PAGE profile of Magrose Strep-Tactin purified Strep-tagII-eGFP protein

 

Partial purchase records (4)

UsernameQuantitybought time
Al***12026-01-05
Ch***12025-11-20
Ra***12025-11-12
Iv***12025-11-06
Total 4 records, divided into1 pages. First Prev Next Last

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