Drug Names

Generic Name: Porcine CCAAT/Enhancer Binding Protein Epsilon (C/EBPE)ELISA Kit

Purpose

Our Porcine CCAAT/Enhancer Binding Protein Epsilon (C/EBPE)ELISA kit is to assay C/EBPE levels in Porcine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to C/EBPE. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for C/EBPE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain C/EBPE and HRP conjugated C/EBPE antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of C/EBPE. You can calculate the concentration of C/EBPE in the samples by comparing the OD of the samples to the standard curve.