The kit is unavaliable, suggest to use Reactive Oxygen Species, ROS Assay Kit Cat No.: AK0696

Drug Names

Generic Name: Bovine Reactive Oxygen Species (ROS)ELISA Kit

Purpose

Our Bovine Reactive Oxygen Species (ROS)ELISA kit is to assay ROS levels in Bovine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ROS. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for ROS is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ROS and HRP conjugated ROS antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ROS. You can calculate the concentration of ROS in the samples by comparing the OD of the samples to the standard curve.