Sunlong Medical™ Rat Leptin (LEP) High Sensitivity ELISA Kit_High Sensitivity ELISA Kit_Sunlong Medical™

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Sunlong Medical™ Rat Leptin (LEP) High Sensitivity ELISA Kit

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Product Summary

Alias:LEP ELISA Kit| OB ELISA Kit| OBS ELISA Kit| Leptin ELISA Kit| Obesity factor ELISA Kit

NO.:HS-EL0285Ra

Storage:2-8℃

Validity:6 months

Assay type:Sandwich Method

Sample Volume:50 μL

Sensitivity:0.075ng/mL

Assay range:0.15625ng/mL-10ng/mL

Species:Rat

Manual

Product Spec:

48T

96T

Number:

Details

Typical Data

The following data and curve are for reference only. The experimenter must establish a standard curve based on their own experimental data.

Standard Conc. (ng/mL)	10	5	2.5	1.25	0.625	0.313	0
OD Value	2.705	1.535	0.795	0.421	0.221	0.133	0.037
Corrected OD Value	2.668	1.498	0.758	0.384	0.184	0.096	0

Recovery

Recovery was tested by spiking known concentrations of Rat LEP into different sample matrices. The recovery range and average recovery are shown below.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=8)	89-99	96
Plasma (n=8)	95-103	98
Cultured Cells (n=8)	96-115	103

Linearity

High concentration Rat LEP was spiked into samples and then serially diluted 2-fold, 4-fold, 8-fold, and 16-fold within the range of the standard curve to assess linearity. Recovery rates and average recovery are shown below.

Dilution		Serum (n=4)	Cultured Cells (n=4)
1:2	Recovery Range (%)	94-112	91-105
1:2	Avg. Recovery (%)	99	101
1:4	Recovery Range (%)	99-114	97-112
1:4	Avg. Recovery (%)	101	107
1:8	Recovery Range (%)	102-119	99-114
1:8	Avg. Recovery (%)	106	111
1:16	Recovery Range (%)	109-119	106-120
1:16	Avg. Recovery (%)	110	118

Citations

1. ILC2 regulates hyperoxia-induced lung injury via an enhanced Th17 cell response in the BPD mouse model

BMC Pulmonary Medicine 2023-05-30

2. Disease-specific corona mediated co-delivery of MTX and siRNA-TNFα by a polypeptide nanoplatform with antigen-scavenging functions in psoriasis

JOURNAL OF CONTROLLED RELEASE 2023-11-02

3. Discovery of novel carboxylesterase 2 inhibitors for the treatment of delayed diarrhea and ulcerative colitis

BIOCHEMICAL PHARMACOLOGY 2023-08-09

4. Glucan from Dictyophora rubrovolvata boosts immunity by regulating AKT-STAT1 pathway to induce dendritic cells maturation and T cells differentiation

Food Frontiers 2023-06-10

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