Sunlong Biotech™ Viral DNA/RNA Kit for Animal (Cat.70416) is suitable for rapid and efficient extraction of viral DNA or RNA from whole blood, serum, plasma, cell-free body fluid, cell culture supernatant virus preservation solution, animal tissue, saliva, swab, environment, etc. The kit is pre-filled, suitable for various types of magnetic rod automatic nucleic acid extractors.

Features

1. Automated, high throughput: 25 mins (up to 96 samples)

2. For multiple complex samples: including blood, tissue, sewage, environment and other complex samples

3. Recovery rate up to 90%

Application Case

1. Performance comparison of different brands of Viral DNA/RNA Kit for Animal

Experimental Samples: add different concentrations of ASFV pseudo virus quality control in blood, saliva, tissue

and sewage samples accordingly.

Experimental method and materials: with Sunlong Biotech™ Rosetta 96, extract ASFV virus nucleic acid from blood,

saliva, tissue and sewage samples using Sunlong Biotech™ Viral DNA/RNA Kit for Animal and competitor kit.

Conclusion: qPCR results show that Sunlong Biotech™ Viral DNA/RNA Kit for Animal has a higher detection rate in

critical concentration or complex samples.

Fig.1 Performance comparison with different brand of Viral DNA/RNA Kit for Animal

2. Client data - a large agricultural and animal husbandry company in Beijing

Experimental Samples: Pig blood, liver, lung, spleen samples

Experimental method and materials: Sunlong BiotechTM Viral DNA/RNA Kit for Animal and competitor, with automatic nucleic acid extraction instrument

Conclusion: The qPCR results show that Ct value of the Sunlong Biotech™ Viral DNA/RNA Kit for Animal is smaller than that of competitor kit in different samples and different ASFV concentrations, also the Ct value is 1-2 smaller in samples with low virus concentration.

Fig.2 Performance comparison of Viral DNA/RNA Kit for Animal using