Sunlong Biotech™ Circulating DNA Kit is using superparamagnetic microspheres and pre-filled buffers to extract circulating DNA from 0.2~5 ml serum or plasma in a fast and efficient way. The quality of the extracted products is stable and reliable, and can be used for PCR amplification, sequencing and detection.

Product Features

1. High Production: The optimized operation procedure to maximize the extraction of circulating DNA;
2. High purity: The optimized buffer system to remove PCR inhibitors and Protein contaminants effectively;
3. Minimum elution volume: Elution volume is about 20μL to ensure higher nucleic acid concentration;
4. Flexible sample volume: extract circulating DNA from 0.2~5 ml serum or plasma.

Application

Recovery rate of DNA fragments from different sample volumes

Add 151bp Nucleic acid fragment into blood to make concentration of 10ng/μL, then use Sunlong Circulating Extraction Kit to recover nucleic acid fragment from different sample volumes and quantify it with qPCR. According to research data, recovery rate of Sunlong Product can exceed 85% in any 0.2~5 mL samples.

 

qPCR amplification curves from the same sample

Add 151bp Nucleic acid fragment into blood to make concentration of 10ng/mL, then use Sunlong Circulating Extraction Kit recover nucleic acid fragment from the sample and quantify it with qPCR. According to research data, Ct values are consistent, which shows this kit has good stability.

 

 Competitor comparison

Circulating DNA obtained from three peripheral blood plasma samples. Then use Qubit to find the concentration. The result shows that Sunlong product has a better performance than the competitor.