Use Sunlong Biotech™ human CD3+ T Cell Isolation Kit to isolate CD3+ T cells from human PBMC, label them with PE anti-human CD3 antibody (clone number OKT3) and then analyze by flow cytometry.

Fig.7 Flow cytometry analysis chart of human PBMC cells isolation by Sunlong BiotechTM Human CD3+ Cell Isoaltion Kit (negative)

Use Sunlong Biotech™ human CD3⁺ T Cell Isolation Kit to isolate CD3⁺ T cells from human PBMC, label them with PE anti-human CD3 antibody (clone number OKT3) and then analyze by flow cytometry.

Conclusion: The purity of CD3⁺ T cells before and after isolation was 69.1% and 97.9% respectively.