Use Sunlong Biotech™ Mouse CD3+ T Cell Isolation Kit to isolate CD3+ T cells from mouse spleen cells, label them with FITC anti-mouse CD3 antibody (clone number 145-2C11), and then analyze by flow cytometry.

Use Sunlong Biotech™ Mouse CD3⁺ T Cell Isolation Kit to isolate CD3⁺ T cells from mouse spleen cells, label them with FITC anti-mouse CD3 antibody (clone number 145-2C11), and then analyze by flow cytometry.

Fig.5 Flow cytometry analysis chart of mouse spleen cells isolation by Sunlong BiotechTM Mouse CD3+ Cell Isolation Kit (negative)

Conclusion: The purity of CD3⁺ T cells before and after isolation was 35.3% and 97.6% respectively.