Use Sunlong Biotech™ Mouse CD4+ T Cell Isolation Kit to isolate CD4+ T cells from mouse spleen cells, label them with FITC anti-mouse CD4 antibody (clone number GK1.5) and then analyze by flow cytometry.

Use Sunlong Biotech™ Mouse CD4⁺ T Cell Isolation Kit to isolate CD4⁺ T cells from mouse spleen cells, label them with FITC anti-mouse CD4 antibody (clone number GK1.5) and then analyze by flow cytometry.

Fig.1 Flow cytometry analysis of mouse spleen cells isolation by Sunlong Biotech^TM Mouse CD4⁺ Cell Isolation Kit (negative selection)

Conclusion: The purity of CD4⁺ T cells before and after isolation was 20.3% and 98.8% respectively.