Use Sunlong Biotech™ Mouse CD8+ T Cell Isolation Kit to isolate CD8+ T cells from mouse spleen cells, label them with FITC anti-mouse CD8 antibody (clone number 53-6.7), and then analyze by flow cytometry.

Use Sunlong Biotech™ Mouse CD8⁺ T Cell Isolation Kit to isolate CD8⁺ T cells from mouse spleen cells, label them with FITC anti-mouse CD8 antibody (clone number 53-6.7), and then analyze by flow cytometry.     

Fig.3 Flow cytometry analysis chart of mouse spleen cells isolation by Sunlong BiotechTM Mouse CD8+ Cell Isolation Kit (negative selection)

Conclusion: The purity of CD8⁺ T cells before and after isolation was 13.8% and 97.1% respectively.