The principle of magnetic beads cell isolation is to use immunomagnetic beads to label cells, the cells bound to the magnetic beads and without bound to the magnetic beads are separated by an external magnetic field. Sunlong Biotech Cell Isolation Kit has two separation technologies, negative selection and positive selection, for both human and mouse samples, and is suitable for different application directions.

(I) Cell Isolation Kit Operation Flow Chart (Positive Selection)

Product Introduction

The positive selection kit uses nano magnetic beads to label and enrich target cells, and then uses a release agent to dissociate the magnetic beads from the cell surface to obtain target cells without magnetic bead labeling.

Features

l Purity reaches more than 95%.

l Wide sample applicability, target cells can be captured directly.

         l Magnetic bead release technology, cells without magnetic beads labeling.

Application Cases

Sunlong Biotech™ Mouse CD4+ Cell Isolation Kit (positive selection)

Use the Sunlong Biotech Mouse CD4+ Cell Isolation Kit (positive selection) to isolate CD4+ cells from spleen cells of BALB/C mice, label them with PE anti-mouse CD4 antibody (clone number RM4-4), and then analyze by flow cytometry.

Fig.5 Flow cytometry analysis chart of mouse spleen cells isolation by Sunlong Biotech Mouse CD4+ Cell Isolation Kit (positive selection)

Conclusion: The purity of CD4+ cells before and after isolation was 13.3% and 97.2% respectively.

Sunlong Biotech™ Mouse CD8+ Cell Isolation Kit (positive selection)

Use Sunlong Biotech Mouse CD8+ Cell Isolation Kit (positive selection) to isolate CD8+ cells from mouse spleen cells of C57BL/6, label them with FITC anti-mouse CD8 antibody (clone number 53-5.8), and then analyze by flow cytometry.

Fig.6 Flow cytometry analysis chart of mouse spleen cells isolation by Sunlong Biotech Mouse CD8+ Cell Isolation Kit (positive selection)

Conclusion: The purity of CD8+ cells before and after isolation was 8.9% and 95.1% respectively.