Human CD66b+ Cell Sorting Kit (Positive Selection) is suitable for CD66b+ cell fraction of human peripheral blood samples CD66b+ cells with up to 98% purity can be obtained by sorting.

Product features:

Simple: no need to separate the column, use with a magnetic separator;

High purity: the purity of cells after sorting can reach 98%;

High activity: The cells are more than 95% active after sorting, and the cell function is good

Assessment data

CD 66b+ cells were sorted from human peripheral blood using a beaver cell sorting kit to analyze the purity, viability, and cell function of the selected CD66b+ cells

1. Cell purity analysis

Figure 1. Flow cytometry detects the purity of CD 66b cells before and after sorting ⁺

CD 66b+ cells were sorted from human peripheral blood using the Human CD66b+ Cell Sorting Kit. Cells before and after sorting were stained with Alexa Fluor 647-labeled anti-CD66b antibody (clone number 6/40c) and then analyzed by flow cytometry, and the purity of CD66b+ cells before and after sorting was 52.62% and 99%, respectively 67%.  

2.  Cell viability analysis

Figure 2. CD 66b+ cell viability before and after flow cytometry sorting

CD 66b+ cells were sorted from human peripheral blood using the Human CD66b+ Cell Sorting Kit. Pre- and post-sorted cells were stained with 7-AAD and cell viability analyzed using flow cytometry. The proportion of viable cells before sorting was 93.45%, and the proportion of viable cells after sorting was 97.18%  

3. Functional detection

 The ability of CD 66b+ cells to be produced under PMA stimulation was detected by sorting CD 66b+ cells from human peripheral blood using the Human CD66b+ Cell Sorting Kit. At 37°C, PMA stimulated the sorted cells for 10 minutes, and the oxidation of the substrate DCFH was detected by flow cytometry to evaluate the ROS production ability in the cells. The results showed that the ROS release level of CD66b+ cells was significantly higher than that in the resting state after PMA stimulation

Figure 3 The release levels of ROS in CD66b+ cells before and after PMA stimulation were analyzed

Combined with the above data, the application of this kit does not require density gradient centrifugation to obtain high-purity neutrophils without affecting cell function.