Anti-DYKDDDDK Magnetic Beads are covalently conjugated with high-quality DYKDDDDK rabbit monoclonal antibodies and nanoscale carboxyl magnetic beads, which can specifically bind to fusion proteins containing DYKDDDDK tags in animal and plant or microbial lysate, serum, ascites, etc., and can be easily applied to DYKDDDDK with the help of magnetic separation equipment such as magnetic racks Immunoprecipitation (IP), Co-IP, or purification of the labeled fusion protein or its protein complex.

Specifications

Product Features:

High load, requiring only 10 ~ 20 μL of beads per experiment

Low background, low non-specificity of magnetic beads

Product Performance Evaluation:

Experimental conditions:

Samples: HEK293T (human embryonic kidney cells) were transfected with Flag-tagged human ILF2 plasmid (Flag-ILF2) for 36 h, and the cells were collected and The corresponding whole cell lysate was obtained by high-speed centrifugation by IP cell lysate.

Magnetic beads: 20 μL;

IP conditions: 500 μL of sample containing approximately 0.7 mg of total protein, magnetic beads incubated overnight with samples at 4°C;

Elution: Add 20 μL of 1 × SDS-PAGE protein loading buffer, boil at 100°C for 5 minutes, and collect the supernatant, i.e., the sample with elution;

Detection: The expression of precipitated Flag-ILF2 was detected by Western blot (WB) method.

 Flag-Tag protein immunoprecipitation experiment