Summary:

Purpose

Our Quick Step Mouse secreted phospholipase A2 ,sPLA2 Elisa Kit is to assay sPLA2  levels in Mouse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to sPLA2 , make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for sPLA2  is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain sPLA2  and HRP conjugated sPLA2  antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of sPLA2  is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS0483Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.