Summary:

Purpose

Our Quick Step Mouse heat shock protein 72 ,HSP-72 ELISA Kit is to assay HSP-72 levels in Mouse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to HSP-72, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HSP-72 is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain HSP-72 and HRP conjugated HSP-72 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of HSP-72 is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS0691Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.