Summary:

Purpose

Our Quick Step Human Lecithin-cholesterol acyltransferase ,lcat ELISA Kit is to assay lcat levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to lcat, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for lcat is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain lcat and HRP conjugated lcat antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of lcat is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS3191Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.