Summary:

Purpose

Our Quick Step Mouse Free Tri-iodothyronine Indes ,Free-T3 ELISA Kit is to assay Free-T3 levels in Mouse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to Free-T3, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Free-T3 is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain Free-T3 and HRP conjugated Free-T3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of Free-T3 is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS0228Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.