Summary:

Purpose

Our is to assay VCAM-1  levels in Mouse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to VCAM-1 , make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for VCAM-1  is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain VCAM-1  and HRP conjugated VCAM-1  antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of VCAM-1  is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS0563Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.