Drug Names

Generic Name: Rat 2,3-Diphosphoglycerate (2,3-DPG)ELISA Kit

Purpose

Our Rat 2,3-Diphosphoglycerate (2,3-DPG)ELISA kit is to assay 2,3-DPG levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 2,3-DPG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for 2,3-DPG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 2,3-DPG and HRP conjugated 2,3-DPG antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 2,3-DPG. You can calculate the concentration of 2,3-DPG in the samples by comparing the OD of the samples to the standard curve.