Drug Names

Generic Name: Rat Complement C1q Tumor Necrosis Factor-Related Protein

12 (CTRP-12)ELISA Kit

Purpose

Our Rat Complement C1q Tumor Necrosis Factor-Related Protein 12 (CTRP-12)ELISA kit is to assay CTRP-12 levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CTRP-12. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for CTRP-12 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CTRP-12 and HRP conjugated CTRP-12 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CTRP-12. You can calculate the concentration of CTRP-12 in the samples by comparing the OD of the samples to the standard curve.