Operation Equipment: Spectrophotometer/microplate reader 

Catalog Number: AK0947-100T-48S

Size:100T/48S

Product Composition: Before use, please carefully check whether the volume of the reagent is consistent with the volume in the bottle.

Product Description:

L-Lactate dehydrogenase (L-LDH or LD) is the terminal enzyme of the glycolysis pathway which is found in nearly all living cells 
(animals, plants, and prokaryotes). L-LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+  to NADH and back.

NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of L-LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.

Note: Before the experiment, it is recommended to select 2-3 sample with large expected differences for pre-experiment.  If  the  absorption  value  of  the   sample  is  not  within  the  measurement  range,  it  is recommended to dilute or increase the sample size for detection

Product Composition: Before use, please carefully check whether the volume of the reagent is consistent with the volume in the bottle.