[KD-Validated] Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody_KO-KD Validated Antibody_Antibody

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[KD-Validated] Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody

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Product Summary

Alias:ACTA2; Actin Alpha 2, Smooth Muscle; ACTSA; Cell Growth-Inhibiting Gene 46 Protein; Actin, Alpha 2, Smooth Muscle, Aorta; Actin, Aortic Smooth Muscle; Alpha-Cardiac Actin; Alpha-Actin-2; EC 3.6.4.-; ACTVS

NO.:KD-10949

Gene name:ACTA2

React Species:Human; Mouse; Rat

Applications:WB; FCM; IHC

UniProt Entry:P62736

NCBI Gene Entry:59

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Product Spec:

20ul

50ul

100ul

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Details

Aliases:	ACTA2; Actin Alpha 2, Smooth Muscle; ACTSA; Cell Growth-Inhibiting Gene 46 Protein; Actin, Alpha 2, Smooth Muscle, Aorta; Actin, Aortic Smooth Muscle; Alpha-Cardiac Actin; Alpha-Actin-2; EC 3.6.4.-; ACTVS
Background:	UniProt Entry: P62736;NCBI Gene Entry: 59
Application Information	Molecular Weight: Predicted, 42 kDa, observed, 42 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB600 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human alpha smooth muscle Actin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of alpha smooth muscle Actin expression in C2C12 cells using alpha smooth muscle Actin antibody 1:2,000. Green, isotype control; red, alpha smooth muscle Actin.

Immunocytochemical staining of C2C12 cells with alpha smooth muscle Actin antibody 1:1,000. Nuclei were stained blue with DAPI; alpha smooth muscle Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-alpha smooth muscle Actin antibody 1:10,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

Western blotting analysis using anti-alpha smooth muscle Actin antibody 1:10,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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