[KD-Validated] Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody_KO-KD Validated Antibody_Antibody

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[KD-Validated] Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody

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Product Summary

Alias:ACTA2; Actin Alpha 2, Smooth Muscle; ACTSA; Cell Growth-Inhibiting Gene 46 Protein; Actin, Alpha 2, Smooth Muscle, Aorta; Actin, Aortic Smooth Muscle; Alpha-Cardiac Actin; Alpha-Actin-2; EC 3.6.4.-; ACTVS

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NO.:KD-10950

Gene name:ACTA2

React Species:Human; Mouse; Rat

Applications:WB; FCM; IHC

UniProt Entry:P62736

NCBI Gene Entry:59

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Product Spec:

20ul

50ul

100ul

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Details

Aliases:	ACTA2; Actin Alpha 2, Smooth Muscle; ACTSA; Cell Growth-Inhibiting Gene 46 Protein; Actin, Alpha 2, Smooth Muscle, Aorta; Actin, Aortic Smooth Muscle; Alpha-Cardiac Actin; Alpha-Actin-2; EC 3.6.4.-; ACTVS
Background:	UniProt Entry: P62736;NCBI Gene Entry: 59
Application Information	Molecular Weight: Predicted, 42 kDa, observed, 42 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB605 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human alpha smooth muscle Actin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of alpha smooth muscle Actin expression in HT-1080 cells using alpha smooth muscle Actin antibody 1:2,000. Green, isotype control; red, alpha smooth muscle Actin.

Immunocytochemical staining of HT-1080 cells with alpha smooth muscle Actin antibody 1:1,000. Nuclei were stained blue with DAPI; alpha smooth muscle Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-alpha smooth muscle Actin antibody 1:10,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

Western blotting analysis using anti-alpha smooth muscle Actin antibody 1:10,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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