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[KD-Validated] Anti-ADK Rabbit Monoclonal Antibody

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Product Summary

Alias:ADK; Adenosine Kinase; AK; Adenosine 5'-Phosphotransferase; EC 2.7.1.20; Testicular Tissue Protein Li 14; EC 2.7.1

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NO.:KD-10047

Gene name:ADK

React Species:Human; Mouse

Applications:WB; IHC

UniProt Entry:P55263

NCBI Gene Entry:132

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Product Spec:

20ul

50ul

100ul

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Details

Aliases:	ADK; Adenosine Kinase; AK; Adenosine 5'-Phosphotransferase; EC 2.7.1.20; Testicular Tissue Protein Li 14; EC 2.7.1
Background:	UniProt Entry: P55263;NCBI Gene Entry: 132
Application Information	Molecular Weight: Predicted, 41 kDa, observed, 46 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB625 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human ADK
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Immunocytochemical staining of HAP-1 cells with ADK antibody 1:1,000. Nuclei were stained blue with DAPI; ADK was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-ADK antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ADK antibody and HRP-conjugated goat anti rabbit secondary antibody respectively.

Western blotting analysis using anti-ADK antibody . ADK expression in wild type (WT) and ADK shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ADK antibody and HRP-conjugated goat anti rabbit secondary antibody respectively.

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