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[KD-Validated] Anti-Acetyl-CoA carboxylase alpha Rabbit Monoclonal Antibody

[KD-Validated] Anti-Acetyl-CoA carboxylase alpha Rabbit Monoclonal Antibody
Total

Product Summary

Alias:ACACA; Acetyl-CoA Carboxylase Alpha; ACC1; ACCA; Acetyl-Coenzyme A Carboxylase Alpha; Acetyl-CoA Carboxylase 1; ACC-Alpha; HACC1; ACAC; ACACalpha; ACCalpha; Acac1; ACC; EC 6.4.1.2; ACACALPHA; ACC-ALPHA; ACCALPHA; ACACAD; ACAC1

NO.:KD-10031

Gene name:ACACA

React Species:Human

Applications:WB; FCM; IHC

UniProt Entry:Q13085

NCBI Gene Entry:31

    Number:

Details

Aliases: ACACA; Acetyl-CoA Carboxylase Alpha; ACC1; ACCA; Acetyl-Coenzyme A Carboxylase Alpha; Acetyl-CoA Carboxylase 1; ACC-Alpha; HACC1; ACAC; ACACalpha; ACCalpha; Acac1; ACC; EC 6.4.1.2; ACACALPHA; ACC-ALPHA; ACCALPHA; ACACAD; ACAC1
Background: UniProt Entry: Q13085;NCBI Gene Entry: 31
Application InformationMolecular Weight: Predicted, 266 kDa, observed, 266 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5645 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Acetyl Coenzyme A Carboxylase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols For general and specific antibody protocols please visit our website. Read all instructions before using this product.
Flow cytometric analysis of Acetyl-CoA carboxylase alpha expression in HepG2 cells using Acetyl-CoA carboxylase alpha antibody 1:2,000. Green, isotype control; red, Acetyl-CoA carboxylase alpha.
Immunocytochemical staining of HepG2 cells with anti-Acetyl-CoA carboxylase alpha antibody 1:1,000. Nuclei were stained blue with DAPI; Acetyl-CoA carboxylase alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Acetyl-CoA carboxylase alpha antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.
Western blotting analysis using anti-Acetyl-CoA carboxylase α antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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