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[KD-Validated] Anti-MLD Rabbit Monoclonal Antibody

[KD-Validated] Anti-MLD Rabbit Monoclonal Antibody
Total

Product Summary

Alias:DEGS1; Delta 4-Desaturase, Sphingolipid 1; DES1; MLD; DEGS-1; FADS7; Cell Migration-Inducing Gene 15 Protein; Sphingolipid Delta(4)-Desaturase DES1; Sphingolipid Delta(4)-Desaturase 1; Dihydroceramide Desaturase 1; Membrane Lipid Desaturase; Retinol Isome

NO.:KD-10301

Gene name:DEGS1

React Species:Human

Applications:WB; FCM; IHC

UniProt Entry:O15121

NCBI Gene Entry:8560

    Number:

Details

Aliases: DEGS1; Delta 4-Desaturase, Sphingolipid 1; DES1; MLD; DEGS-1; FADS7; Cell Migration-Inducing Gene 15 Protein; Sphingolipid Delta(4)-Desaturase DES1; Sphingolipid Delta(4)-Desaturase 1; Dihydroceramide Desaturase 1; Membrane Lipid Desaturase; Retinol Isomerase; Des-1; Degenerative Spermatocyte Homolog 1, Lipid Desaturase (Drosophila); Degenerative Spermatocyte Homolog 1, Lipid Desaturase; Degenerative Spermatocyte Homolog, Lipid Desaturase; Membrane Fatty Acid (Lipid) Desaturase; Degenerative Spermatocyte Homolog 1; Migration-Inducing Gene 15 Protein; Sphingolipid Delta 4 Desaturase; Dihydroceramide Desaturase-1; EC 1.14.19.17; EC 5.2.1.-; HLD18; MIG15; DES-1; DEGS
Background: UniProt Entry: O15121;NCBI Gene Entry: 8560
Application InformationMolecular Weight: Predicted, 38 kDa, observed, 34 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1215 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human MLD
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols For general and specific antibody protocols please visit our website. Read all instructions before using this product.
Flow cytometric analysis of MLD expression in HepG2 cells using MLD antibody 1:2,000. Green, isotype control; red, MLD.
Immunocytochemical staining of HT-1080 cells with MLD antibody 1:1,000. Nuclei were stained blue with DAPI; MLD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-MLD antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.
Western blotting analysis using anti-MLD antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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