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[KD-Validated] Anti-JUP Rabbit Monoclonal Antibody

[KD-Validated] Anti-JUP Rabbit Monoclonal Antibody
Total

Product Summary

Alias:JUP; Junction Plakoglobin; DP3; DPIII; CTNNG; PDGB; PKGB; PG; Catenin (Cadherin-Associated Protein), Gamma 80kDa; Desmosomal Protein 3; Desmoplakin III; Desmoplakin-3; Catenin Gamma

NO.:KD-10520

Gene name:JUP

React Species:Human; Mouse

Applications:WB; FCM; IHC

UniProt Entry:P14923

NCBI Gene Entry:3728

    Number:

Details

Aliases: JUP; Junction Plakoglobin; DP3; DPIII; CTNNG; PDGB; PKGB; PG; Catenin (Cadherin-Associated Protein), Gamma 80kDa; Desmosomal Protein 3; Desmoplakin III; Desmoplakin-3; Catenin Gamma
Background: UniProt Entry: P14923;NCBI Gene Entry: 3728
Application InformationMolecular Weight: Predicted, 82 kDa, observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6055 Spcies Reaceivity: Human, Mouse Applicseions Tsted: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Catenin gamma
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols For general and specific antibody protocols please visit our website. Read all instructions before using this product.
Western blotting analysis using anti-JUP antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:2,000. Green, isotype control; red, JUP.
Immunocytochemical staining of HepG2 cells with anti-JUP antibody 1:1,000. Nuclei were stained blue with DAPI; JUP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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