Details
Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell..
**1.Introduction**
This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 3fg/μL level. This kit can be used in combination with our Magnetic Residual DNA Sample Preparation Kit (Cat#CG-HCD100T). The preparation process of DNA Control is completely consistent with National Standard, therefore it has high purity and no protein and ion interference. DNA Control has been calibrated by National Standard to ensure the accuracy of the sample quantitative detection.
The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.
**2. MATERIALS**
DNA Amplification |
|||
Components NO. |
Components Name |
Size |
|
50T |
100T |
||
B1 |
2XqPCR Mix |
0.625mL |
1.25mL |
B2 |
Primer&Probe Mix |
100μL |
200μL |
B3 |
2×1.5mL |
4×1.5mL |
|
B4 |
DNA Control (30ng/μL) |
25μL |
50μL |
B5 |
RNase-Free H2O |
0.5mL |
1mL |
B6 |
50X ROX Reference Dye(Optional) |
0.15ml |
0.3ml |
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